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Registro Completo |
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Biblioteca(s): |
Embrapa Semiárido. |
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Data corrente: |
15/03/2000 |
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Data da última atualização: |
10/03/2023 |
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Autoria: |
SANTOS, I. R. I. |
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Título: |
Desiccation and freezing tolerance of embryonic axes and lateral buds of Citrus spp: implication for germplasm conservation. |
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Ano de publicação: |
1999 |
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Fonte/Imprenta: |
Fort Collins: Colorado State University, 1999. |
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Páginas: |
159p. |
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Idioma: |
Inglês |
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Notas: |
Ph.D. Thesis. |
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Conteúdo: |
Citrus is the second most importan fruit crop in the world in terms of economic value. Germplasm conservation of commercial cultivars as well as of wild relatives is a priority. Presently, Citrus germplasm has been preserved mainly in field gene banks. Plants maintained in the field are susceptible to biological and climati disasters. Cryopreservation (conservation in liquid nitrogen at -196oC or in its vapor phase at - 150oC) can in theory ensure storage of plant material for several years maintaining high biological and genetic integrity. This research examined several aspects of tolerance to desiccation and freezing in an attempt to develop cryopreseravation protocols for embryonic axes and lateral buds of Pineapple sweet orange (Citrus sinensis [L.] Osb.) and for embryonic axes of Citrus limon Burm. F. and Citrus reticulata Blanco. The roles of alginate encapsulation and pre-culture with sucrose in enhancing tolerance of freezing in liquid nitrogen were tested. Carbohydrate analysis was carried out on axes pre-cultured with sucrose or not and on buds from cold acclimated plants to gain insight on the role of sucrose as a cryopreservation stabilizer. The interrrelationshisp between water content and thermodynamic properties of water during freezing and thawing, which are critical in achieving survival in liquid nitrogen, were also studied. Prior to cryopreservation studies the germiantion of isolated embryonic axes was assessed. For that, embryonic axes with and without alginate encapsulation and with and without sucrose pre-culture, but without dehydration or exposure to liquid nitrogen, were cultured in vitro. Total seedling recovery was observed whether or not axes had been encapsulated and whether normal seedlings, without intermediary callus formation, within three weeks of culture in vitro. MenosCitrus is the second most importan fruit crop in the world in terms of economic value. Germplasm conservation of commercial cultivars as well as of wild relatives is a priority. Presently, Citrus germplasm has been preserved mainly in field gene banks. Plants maintained in the field are susceptible to biological and climati disasters. Cryopreservation (conservation in liquid nitrogen at -196oC or in its vapor phase at - 150oC) can in theory ensure storage of plant material for several years maintaining high biological and genetic integrity. This research examined several aspects of tolerance to desiccation and freezing in an attempt to develop cryopreseravation protocols for embryonic axes and lateral buds of Pineapple sweet orange (Citrus sinensis [L.] Osb.) and for embryonic axes of Citrus limon Burm. F. and Citrus reticulata Blanco. The roles of alginate encapsulation and pre-culture with sucrose in enhancing tolerance of freezing in liquid nitrogen were tested. Carbohydrate analysis was carried out on axes pre-cultured with sucrose or not and on buds from cold acclimated plants to gain insight on the role of sucrose as a cryopreservation stabilizer. The interrrelationshisp between water content and thermodynamic properties of water during freezing and thawing, which are critical in achieving survival in liquid nitrogen, were also studied. Prior to cryopreservation studies the germiantion of isolated embryonic axes was assessed. For that, embryonic axes with and without a... Mostrar Tudo |
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Palavras-Chave: |
Citro; Citrus reticula; Citrus spp; Congelamento Tolerancia; Conservation; Cryopreservatio; Desiccation; Dessidratacao; Embriogenesis; Organges; Plant laboratory; Protocolo; Tolerance. |
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Thesagro: |
Conservação; Criopreservação; Frio; Gema; Germoplasma; Laboratório; Laranja; Limão; Planta. |
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Thesaurus Nal: |
buds; freezing; germplasm; lemons. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02965nam a2200445 a 4500
001 1149518
005 2023-03-10
008 1999 bl uuuu m 00u1 u #d
100 1 $aSANTOS, I. R. I.
245 $aDesiccation and freezing tolerance of embryonic axes and lateral buds of Citrus spp$bimplication for germplasm conservation.
260 $aFort Collins: Colorado State University$c1999
300 $a159p.
500 $aPh.D. Thesis.
520 $aCitrus is the second most importan fruit crop in the world in terms of economic value. Germplasm conservation of commercial cultivars as well as of wild relatives is a priority. Presently, Citrus germplasm has been preserved mainly in field gene banks. Plants maintained in the field are susceptible to biological and climati disasters. Cryopreservation (conservation in liquid nitrogen at -196oC or in its vapor phase at - 150oC) can in theory ensure storage of plant material for several years maintaining high biological and genetic integrity. This research examined several aspects of tolerance to desiccation and freezing in an attempt to develop cryopreseravation protocols for embryonic axes and lateral buds of Pineapple sweet orange (Citrus sinensis [L.] Osb.) and for embryonic axes of Citrus limon Burm. F. and Citrus reticulata Blanco. The roles of alginate encapsulation and pre-culture with sucrose in enhancing tolerance of freezing in liquid nitrogen were tested. Carbohydrate analysis was carried out on axes pre-cultured with sucrose or not and on buds from cold acclimated plants to gain insight on the role of sucrose as a cryopreservation stabilizer. The interrrelationshisp between water content and thermodynamic properties of water during freezing and thawing, which are critical in achieving survival in liquid nitrogen, were also studied. Prior to cryopreservation studies the germiantion of isolated embryonic axes was assessed. For that, embryonic axes with and without alginate encapsulation and with and without sucrose pre-culture, but without dehydration or exposure to liquid nitrogen, were cultured in vitro. Total seedling recovery was observed whether or not axes had been encapsulated and whether normal seedlings, without intermediary callus formation, within three weeks of culture in vitro.
650 $abuds
650 $afreezing
650 $agermplasm
650 $alemons
650 $aConservação
650 $aCriopreservação
650 $aFrio
650 $aGema
650 $aGermoplasma
650 $aLaboratório
650 $aLaranja
650 $aLimão
650 $aPlanta
653 $aCitro
653 $aCitrus reticula
653 $aCitrus spp
653 $aCongelamento Tolerancia
653 $aConservation
653 $aCryopreservatio
653 $aDesiccation
653 $aDessidratacao
653 $aEmbriogenesis
653 $aOrganges
653 $aPlant laboratory
653 $aProtocolo
653 $aTolerance
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Embrapa Semiárido (CPATSA) |
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| Registros recuperados : 590 | |
| 5. |  | SANTOS, I. R. I. Criopreservação de sementes. In: CONGRESSO BRASILEIRO DE RECURSOS GENÉTICOS; WORKSHOP EM BIOPROSPECÇÃO E CONSERVAÇÃO DE PLANTAS NATIVAS DO SEMI-ÁRIDO, 3.; WORKSHOP INTERNACIONAL SOBRE BIOENERGIA E MEIO AMBIENTE, 2010, Salvador. Bancos de germoplasma: descobrir a riqueza, garantir o futuro: anais. Brasília, DF: Embrapa Recursos Genéticos e Biotecnologia, 2010.| Tipo: Nota Técnica/Nota Científica |
| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| Registros recuperados : 590 | |
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